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Because of the nature of phage display, an antibody can be easily screened for its specificity against a specific target to identify antibodies with high affinity. However, in the case of panning, the antibody needs to be captured by the target antigen. In this case, the target antigen is determined through molecular docking, which can be verified by chemical, structural, or biophysical techniques. For example, the target antigen can be purified and used as an antigen to identify the antibody by panning or phage display methods. Alternatively, the antibody can be expressed by recombinant techniques and analyzed by biochemical methods. In addition, the antibody can be tagged with a fluorescent dye or a radioisotope for imaging or radioimmunoassay (RIA) purposes, respectively.
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